Cover Picture: A visualized automatic particle counting strategy for single‐cell level telomerase activity quantification (View 3/2023)

Cover Picture.

Automatic platelet counting with the Couler particle counter.

A method for accurately counting platelets is described using the Coulter counter model B fitted with a standard 100mu aperture tube. This enables the counter to be used for red and white cell as well as platelet counts using the same aperture tube. The method uses standard equipment except for a small inexpensive electronic speed controller.

Kras mutations increase telomerase activity and targeting telomerase is a promising therapeutic strategy for Kras-mutant NSCLC

As shortened telomeres inhibit tumor formation and prolong life span in a KrasG12D mouse lung cancer model, we investigated the implications of telomerase in Kras-mutant NSCLC. We found that Kras mutations increased TERT (telomerase reverse transcriptase) mRNA expression and telomerase activity and telomere length in both immortalized bronchial epithelial cells (BEAS-2B) and lung adenocarcinoma...

A Modified Discreet Particle Swarm Optimization for a Multi-level Emergency Supplies Distribution Network

Currently, the research of emergency supplies distribution and decision models mostly focus on deterministic models and exact algorithm. A few of studies have been done on the multi-level distribution network and matheuristic algorithm. In this paper, random processes theory is adopted to establish emergency supplies distribution and decision model for multi-level network. By analyzing the char...

Alternative Splicing of hTERT Pre-mRNA: A Potential Strategy for the Regulation of Telomerase Activity

The activation of telomerase is one of the key events in the malignant transition of cells, and the expression of human telomerase reverse transcriptase (hTERT) is indispensable in the process of activating telomerase. The pre-mRNA alternative splicing of hTERT at the post-transcriptional level is one of the mechanisms for the regulation of telomerase activity. Shifts in splicing patterns occur...